Coding

Part:BBa_K195300

Designed by: Tung Chao   Group: iGEM09_NYMU-Taipei   (2009-10-21)


neuD,neuB,neuA,neuC,neuE(neu gene cluster).

The coding sequence neu genes are operate in one operon in E.coli K1, which are coding for the enzymes that are involved in sialic acid synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 4521
    Illegal EcoRI site found at 4576
    Illegal XbaI site found at 2941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 4521
    Illegal EcoRI site found at 4576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 4521
    Illegal EcoRI site found at 4576
    Illegal BglII site found at 4934
    Illegal BglII site found at 5105
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 4521
    Illegal EcoRI site found at 4576
    Illegal XbaI site found at 2941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 4521
    Illegal EcoRI site found at 4576
    Illegal XbaI site found at 2941
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1870


[edit]
Categories
Parameters
None